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2Flo Ventures

Venture capital

Chicago · Pre-Seed–Seed · 45 people tracked

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Investment thesis

2Flo Ventures invests in healthcare and life science solutions aimed at improving quality of life, expanding access to care, and advancing safe and effective treatments. The firm backs determined entrepreneurs tackling large unmet medical needs, combining decades of experience across healthcare and entrepreneurship to support and grow its portfolio of life science companies.

Source: firm website, verified Aug 3, 2026.

Stage
Pre-Seed–Seed
Check
$400 – $1K
HQ
Chicago, IL, US
Leads rounds
Follows
Instruments
Equity
Founded
2020
Tracked investments
10

Team · 45 people

from the firm's website
Charlotte Jones-Burton· Partner

A physician leader with expertise in clinical development, health equity, and healthcare innovation.

Amanda Johnson· Venture Fellow
Angela James· Senior Advisorin
Brenda Ametepe· Venture Fellow
Harrison Esterly· Venture Fellow
Helen Wang· Venture Fellow
Ibraheem Alinur· Vice Presidentin

An emerging global health leader focused on tackling health disparities and preventable diseases.

Jaysón Davidson· Venture Fellow
Modestus Obochi· Senior Advisorin
Patrick Walsh· Finance Advisorin
Richard Hubbard· Strategic Advisorin
Rose Blackburne· Senior Advisorin
Rukan Parves· Venture Fellow
Shalise Burch· Venture Fellow
Ta Thanakornyothin· Venture Fellow
Violet Wu· Venture Fellow

Founder mafia

4 people who came through 2Flo Ventures went on to found or lead other companies.

What they invest in

from 9 of 10 portfolio companies

Healthtech ×9Biotech ×6Digital Health ×4SaaS ×3Medical Devices ×2AI & Machine Learning ×1Deep Tech ×1

HQs:United States×7

Investments · 10 tracked

founders shown can be your intro path

By year:2019 · 12016 · 12 dated

Vivacelle Biovivacellebio.comin✓ verified

Vivacelle Bio develops phospholipid nanoparticle therapies (VBI-S and VBI-1) that restore intravascular volume and redirect excess nitric oxide to treat hypovolemia in patients with septic shock or severe blood loss.

BiotechHealthtechMedical DevicesChicago, US

Cuthbert Ormand Simpkins(Executive Officer, Director)Diane Simpkins(Co-Founder & Treasurer, Board of Directors)inConrad Epting(Founding Member / Scientific Consultant)inHarven DeShield(Executive Officer, Director)inStephen Keith(Executive Officer, Director)Paul(CEO/CFO)

4D Healthware4dhealthware.cominJul 2016

4D Healthware offers Virtual CCM, a technology platform for chronic care management that lets healthcare providers deliver care beyond the clinic in settings such as homes, pharmacies, grocery stores, workplaces, and online.

AI & Machine LearningDigital HealthHealthtechSaaSChicago, US

Star Cunningham(Founder)

Co-investorsPortfolia

Akan Biosciencesakanbio.com

AKAN Biosciences is a biotechnology and cellular therapy company developing injectable, adipose stem cell-based regenerative products to treat degenerative diseases and injuries.

BiotechHealthtechMedical Devices

CancerIQcanceriq.comin𝕏

CancerIQ is a precision health software platform that helps health systems run cancer risk assessment, screening, genetic testing, and patient navigation programs, with tools for risk stratification, automated documentation, care plan management, and EHR integration.

Digital HealthHealthtechSaaS

Feyi Ayodele(Founder)in

Co-investorsCleveland AvenueHealthX VenturesMcKesson VenturesOSF VenturesImpact Engine

Delphian Therapeuticsdelphiantx.com

Delphian Tx is a clinical-stage biopharmaceutical company developing cannabinoid-based drugs (CBD:THC combinations) targeting the endocannabinoid system to treat migraine and other complex pain and CNS disorders.

BiotechHealthtech

KeyCarekeycare.orgin𝕏

KeyCare builds virtual care technology and telehealth solutions to extend health system capacity and improve access to healthcare.

Digital HealthHealthtechSaaSChicago, US

Lyle Berkowitz, MDinSulabh Agarwal(Executive Officer, Director)inCarrie Nelson, MDinMike RobbinsinAndy JacksonJennifer Timm

Co-investors8VCBold Capital PartnersHealthX VenturesCultivation CapitalLRVHealth

ResQ Pharmaresqpharma.com

ResQ Pharma is a pharmaceutical company developing LipidRescue (LRT), an investigational lipid-based drug therapy that is pending FDA approval.

BiotechHealthtechGlenview, US

Guy Weinberg(Director, President and Secretary of Issuer)Dr. Weinberg(Founder and President)August H. Schupp(Director, CAO and General Counsel of Issuer)Dominic O'Keefe(Director and COO of Issuer)David Bowman(Executive Officer)Dr. Mukesh Kumar(Director of Regulatory Affairs)in

Temprian Oncologytemprianonc.com

Temprian Oncology develops nanoparticle-based "Supercarriers" that deliver a prodrug toxic to melanoma cells, releasing the active ingredient inside metastatic melanoma tumor cells in low-pH pigment-making organelles.

BiotechDeep TechHealthtech

Temprian Therapeuticstempriantherapeutics.comin𝕏Jan 2019

Temprian Therapeutics is a biopharmaceutical company developing an HSP70i-based immunotherapy for vitiligo.

BiotechDigital HealthHealthtechChicago, US

Isabelle Caroline Le Poole(Executive Officer, Director)Caroline Le Poole(CSO & Co-Founder)inKettil Cedercreutz(Board Member & Co-Founder)inSumeet Dagar(CEO)Paul Burton(CFO & Board Member)Modestus Obochi(Commercialization & Board Member)

Vitiligo treatment biotech

Biotech company developing safe and effective treatment for the skin depigmentation disease vitiligo.

Frequently asked questions

How many investments has 2Flo Ventures made?
2Flo Ventures has 10 tracked investments.
What stage does 2Flo Ventures invest at?
2Flo Ventures invests at the Pre-Seed, Seed stages.
What is 2Flo Ventures's typical check size?
2Flo Ventures typically writes checks of $400 – $1K.
What sectors does 2Flo Ventures focus on?
2Flo Ventures focuses on Biotech, Digital Health, Healthtech, Impact & Social Good, Medical Devices, Sector Agnostic.
Where is 2Flo Ventures located?
2Flo Ventures is headquartered in Chicago, US.
Does 2Flo Ventures lead rounds?
2Flo Ventures typically participates in rounds rather than leading them.
Who does 2Flo Ventures co-invest with?
2Flo Ventures most often shares deals with HealthX Ventures, 8VC, Bold Capital Partners, among 7 tracked co-investors.

Images

2Flo Ventures photo2Flo Ventures photo2Flo Ventures photo2Flo Ventures photo

Co-investors · 1

GenNx360 Capital Partners

GenNx360 is a growth equity and operations-focused firm that invests exclusively in North American business-to-business industrial services and manufacturing companies in fragmented markets with structural tailwinds, providing operational expertise and transaction experience to drive value creation and sustainable growth.

Firms observed investing alongside 2Flo Ventures, from public investor directories. Shared companies are the overlap with 2Flo Ventures's recorded portfolio.

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