2Flo Ventures
Venture studioChicago · Pre-Seed–Seed · 46 people tracked
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Investment thesis
2Flo Ventures backs determined entrepreneurs building life sciences and healthcare solutions that address large, unmet medical needs. As a combined startup studio and venture capital firm, it emphasizes impactful innovation in healthcare, with a recurring focus on reducing health disparities and improving access to care for underserved and minority populations. Its portfolio spans biotech therapeutics, precision oncology, regenerative medicine, virtual and digital health platforms, and treatments for conditions ranging from vitiligo and metastatic melanoma to drug overdose and shock.
Source: firm website, verified Sep 27, 2026.
Team · 46 people
from the firm's website & curatedFounder mafia
3 people who came through 2Flo Ventures went on to found or lead other companies.
What they invest in
from 9 of 9 portfolio companiesHealthtech ×9Biotech ×6Digital Health ×4SaaS ×3Medical Devices ×2AI & Machine Learning ×1Deep Tech ×1Enterprise Software ×1
HQs:United States×7
Investments · 9 tracked
founders shown can be your intro pathBy year:2024 · 12019 · 12016 · 13 dated
Vivacelle Bio develops phospholipid nanoparticle therapies (VBI-S and VBI-1) that restore intravascular volume and redirect excess nitric oxide to treat hypovolemia in patients with septic shock or severe blood loss.
BiotechHealthtechMedical DevicesChicago, US
Cuthbert Ormand Simpkins(Executive Officer, Director)Diane Simpkins(Co-Founder & Treasurer, Board of Directors)inConrad Epting(Founding Member / Scientific Consultant)inHarven DeShield(Executive Officer, Director)inPaul(CEO/CFO)Stephen N. Keith(Former CEO)in
4D Healthware offers Virtual CCM, a technology platform for chronic care management that lets healthcare providers deliver care beyond the clinic in settings such as homes, pharmacies, grocery stores, workplaces, and online.
AI & Machine LearningDigital HealthHealthtechSaaSChicago, US
Star Cunningham(Founder)in
Co-investorsPortfolia
AKAN Biosciences is a biotechnology and cellular therapy company developing injectable, adipose stem cell-based regenerative products to treat degenerative diseases and injuries.
BiotechHealthtechMedical Devices
CancerIQ is a healthcare software platform that helps hospitals and providers run cancer risk-assessment and precision prevention programs by gathering patient data, triaging patients into risk tiers using guidelines like NCCN and Tyrer-Cuzick, and managing genetic counseling, testing, and ongoing care.
Digital HealthHealthtechSaaSChicago, US
Olufeyikemi Olopade Ayodele(Executive Officer, Director)Feyi Ayodele(Founder)inHaibo Lu(Executive Officer, Director)Christopher Bun(Executive Officer)Jordan Dolin(Director)Evan Wood(Director)
Co-investorsCleveland AvenueHealthX VenturesLightbankMcKesson VenturesMerck Global Health Innovation FundMSD Global Health Innovation Fund+2 more
Delphian Tx is a clinical-stage biopharmaceutical company developing cannabinoid-based drugs (CBD:THC combinations) targeting the endocannabinoid system to treat migraine and other complex pain and CNS disorders.
BiotechHealthtech
KeyCare builds virtual care technology and telehealth solutions to extend health system capacity and improve access to healthcare.
Digital HealthEnterprise SoftwareHealthtechSaaSChicago, US
Lyle Berkowitz(Founder)inSulabh Agarwal(Executive Officer, Director)inCarrie Nelson, MDinMike Robbins(Executive Officer)inAndy JacksoninJennifer Timmin
Co-investors8VCBold Capital PartnersCorewell Health VenturesHealthX VenturesMarram VenturesCultivation Capital+1 more
ResQ Pharma is a pharmaceutical company developing LipidRescue (LRT), an investigational lipid-based drug therapy that is pending FDA approval.
BiotechHealthtechGlenview, US
Guy Weinberg(Director, President and Secretary of Issuer)inDr. Weinberg(Founder and President)inAugust H. Schupp(Director, CAO and General Counsel of Issuer)Dominic O'Keefe(Director and COO of Issuer)David Bowman(Executive Officer)Dr. Mukesh Kumar(Director of Regulatory Affairs)in
Temprian Oncology develops nanoparticle-based "Supercarriers" that deliver a prodrug toxic to melanoma cells, releasing the active ingredient inside metastatic melanoma tumor cells in low-pH pigment-making organelles.
BiotechDeep TechHealthtech
Temprian Therapeutics is a biopharmaceutical company developing an HSP70i-based immunotherapy for vitiligo.
BiotechDigital HealthHealthtechChicago, US
Isabelle Caroline Le Poole(Executive Officer, Director)Caroline Le Poole(CSO & Co-Founder)inSumeet Dagar(CEO)inPaul Burton(CFO & Board Member)inJohn Nieland(Chief Innovation Officer)inKettil Cedercreutz(Board Member & Co-Founder)in
Portfolio news
latest coverage across their companiesKeyCare · finsmes.com · Mar 3, 2026
Frequently asked questions
- How many investments has 2Flo Ventures made?
- 2Flo Ventures has 9 tracked investments.
- What stage does 2Flo Ventures invest at?
- 2Flo Ventures invests at the Pre-Seed, Seed stages.
- What is 2Flo Ventures's typical check size?
- 2Flo Ventures typically writes checks of $1K.
- What sectors does 2Flo Ventures focus on?
- 2Flo Ventures focuses on Biotech, Digital Health, Healthtech, Impact & Social Good, Medical Devices, Sector Agnostic.
- Where is 2Flo Ventures located?
- 2Flo Ventures is headquartered in Chicago, US.
- Does 2Flo Ventures lead rounds?
- 2Flo Ventures typically participates in rounds rather than leading them.
- Who does 2Flo Ventures co-invest with?
- 2Flo Ventures most often shares deals with HealthX Ventures, 8VC, Bold Capital Partners, among 12 tracked co-investors.
Images
Co-investors · 1
GenNx360 Capital Partners is a private equity firm investing in North America-based B2B middle-market industrial services and manufacturing companies in fragmented markets, driving value through operational engagement and a buy-and-build strategy.
Firms observed investing alongside 2Flo Ventures, from public investor directories. Shared companies are the overlap with 2Flo Ventures's recorded portfolio.
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