🦊/Companies

Kytopen

Cambridge, US · Founded 2017 · Delaware corporation · 10 known investors

Find your way into Kytopen

1,866 people in our graph share verified history with the Kytopen team — schools, employers, funds. One of them is your warm intro.

Wesley Chanunlockedknows Michael Chiu · together at Massachusetts Institute of Technology (overlapped)
×5knows the team · via Massachusetts Institute of Technology
×2knows the team · via Stanford University
knows the team · via MIT Sloan School of Management
knows the team · via Virginia Tech
knows the team · via Massachusetts Institute of Technology (MIT)

Kytopen develops non-viral gene delivery technology called Flowfect that combines fluid flow with electric fields to deliver payloads such as mRNA, DNA, and CRISPR Cas9 RNP into cells for high-throughput transfection. Its platforms serve cell therapy researchers and developers, supporting drug discovery, process optimization, and scaling from small-volume experiments to manufacturing.

Founders & leadership

Kytopen was founded in 2017 by Cullen Buie and Paulo Garcia.

CBCullen Buie
Cullen BuieinCo-FounderCullen Buie is director of Kytopen and has research expertise in electrokinetics, electrochemistry, fluid mechanics, and microscale engineering applied to biotechnology.
PGPaulo Garcia
Paulo GarciainCo-Founder & AdvisorPaulo Garcia holds a Ph.D. in biomedical engineering from Virginia Tech - Wake Forest University, where his research focused on pulsed electric field applications for minimally invasive treatment. He completed postdoctoral training and led biomanufacturing research in the Mechanical Engineering department at MIT, where he co-founded Kytopen to develop non-viral gene delivery technology; he later became CEO and President of BLASTID, a Harvard University spinout developing diagnostic platforms from the ARPA-H DARTS program.
MCMichael Chiu
Michael ChiuinChief Executive OfficerMichael Chiu founded and previously led a VC-backed startup through a successful exit, and has held general management roles at large technology companies including oversight of a business unit generating over $60M in revenue. He brings experience in product development, commercialization, and cross-functional operations including engineering, supply chain, and marketing.
JV
Julio VegaMr. Vega is the Secretary of the Issuer.

Board

PMPaxton Major
Paxton MajorinBoard Director2023–2026Managing Director at Northpond Ventures
ADAnn DeWitt
Ann DeWittinBoard directorGeneral Partner at The Engine Ventures
PW
Paul WottonBoard director

Investors · 10

Also in the syndicate · 1

The Engine

Funding

SEC filings, press & company announcements

$52.7M disclosed across 5 of 6 rounds · 2019–2026

$7.1MraisedFeb 2025 · 13 investors · Biotechnology
Rule 506(b)
Officers, directors & promoters on the filing
  • Cullen BuieDirector
  • Julio VegaExecutive Officer
  • Michael ChiuDirector, Executive Officer
  • Paul WottonDirector
  • Paxton MajorDirector
Offering amount
$15M
Amount sold
$7.1M
First sale
Feb 2025
Incorporated
Corporation, Delaware
Federal exemptions
06b
Full filing on SEC EDGAR ↗
$5.7MraisedFeb 2024 · 22 investors · Biotechnology
Rule 506(b)
Officers, directors & promoters on the filing
  • Paxton MajorDirector
  • Michael ChiuDirector, Executive Officer
  • Bethany GrantExecutive Officer
  • Edward FreedmanDirector
  • Cullen BuieDirector
  • Paul WottonDirector
Offering amount
$8M
Amount sold
$5.7M
First sale
Jan 2024
Incorporated
Corporation, Delaware
Federal exemptions
06b
Full filing on SEC EDGAR ↗
$32.5MraisedOct 2021 · 18 investors · Biotechnology
Rule 506(b)
Officers, directors & promoters on the filing
  • Adam WieschhausDirector
  • Paulo GarciaExecutive Officer, Director, Promoter
  • Theresa TribbleDirector
  • Cullen BuieDirector
Offering amount
$32.5M
Amount sold
$32.5M
First sale
Sep 2021
Incorporated
Corporation, Delaware, 2017
Federal exemptions
06b
Full filing on SEC EDGAR ↗
$3.1MraisedNov 2020 · 11 investors · Biotechnology
Rule 506(b)
Officers, directors & promoters on the filing
  • Ann DeWittDirector
  • Paulo GarciaExecutive Officer, Director, Promoter
  • Cullen BuieDirector
Offering amount
$4M
Amount sold
$3.1M
First sale
Oct 2020
Incorporated
Corporation, Delaware, 2017
Federal exemptions
06b
Full filing on SEC EDGAR ↗
$4.3MraisedMay 2019 · 9 investors · Biotechnology
Rule 506(b)
Officers, directors & promoters on the filing
  • Paulo GarciaExecutive Officer, Director, Promoter
  • Julio VegaExecutive Officer
  • Cullen BuieDirector
  • Ann DeWittDirector
Offering amount
$4.3M
Amount sold
$4.3M
First sale
May 2019
Incorporated
Corporation, Delaware, 2017
Federal exemptions
06b
Full filing on SEC EDGAR ↗

Source: SEC EDGAR Form D. Amounts as filed; amended filings shown once at their latest values.

Non-dilutive funding · 3 SBIR/STTR awards

Federal grants — no equity taken
AgencyPhaseYearAmount
National Institutes of Health (NIH)National Institutes of HealthPhase I2021$300K
National Science FoundationPhase II2019$948.2K
National Science FoundationPhase I2018$225K

Source: SBIR.gov award data (U.S. Small Business Administration). SBIR/STTR awards are competitive federal R&D grants and contracts — non-dilutive capital alongside any venture rounds above.

In the news

Frequently asked questions

What does Kytopen do?
Kytopen develops non-viral gene delivery technology called Flowfect that combines fluid flow with electric fields to deliver payloads such as mRNA, DNA, and CRISPR Cas9 RNP into cells for high-throughput transfection. Its platforms serve cell therapy researchers and developers, supporting drug discovery, process optimization, and scaling from small-volume experiments to manufacturing.
Who founded Kytopen?
Kytopen was founded by Cullen Buie, Paulo Garcia in 2017.
Who are Kytopen's investors?
Kytopen's investors include Bantam Group, Horizons Ventures, MassVentures, Selvedge Venture, TBD Angels, Telegraph Hill Partners, The Engine Ventures, Alexandria and 1 more.
How much funding has Kytopen raised?
Kytopen has disclosed $52.7M raised across 5 of its 6 known rounds.
Where is Kytopen headquartered?
Kytopen is headquartered in Cambridge, US.